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restriction enzyme sacii  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzyme sacii
    Restriction Enzyme Sacii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/restriction+enzymes+sacii/Sac+II/10__1016_slash_j__scr__2026__103973-43-27-30
    Average 96 stars, based on 862 article reviews
    restriction enzyme sacii - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Continued selection on cryptic SARS-CoV-2 observed in Missouri wastewater.
    Article Snippet: .. The Δ19 spike clone with the D614G mutation, as previously described [10], was used as a starting template to generate the 22-10-04 spike and was digested using the restriction enzymes SacII and XhoI (New England Biolabs). .. The 12 missense mutations found in 22-10-04 were designed using A Plasmid Editor (ApE [11]), ordered as a gBlock (Integrated DNA Technologies), and inserted into the D614G Δ19 spike vector at the SacII and XhoI sites using the 5x InFusion Snap Assembly Master Mix (Takara Bio).

    Article Title: Continued selection on cryptic SARS-CoV-2 observed in Missouri wastewater
    Article Snippet: .. The Δ19 spike clone with the D614G mutation, as previously described [ ], was used as a starting template to generate the 22-10-04 spike and was digested using the restriction enzymes SacII and XhoI (New England Biolabs). .. The 12 missense mutations found in 22-10-04 were designed using A Plasmid Editor (ApE [ ]), ordered as a gBlock (Integrated DNA Technologies), and inserted into the D614G Δ19 spike vector at the SacII and XhoI sites using the 5x In-Fusion Snap Assembly Master Mix (Takara Bio).

    other:


    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. Both PCR products were assembled using the Gibson assembly protocol (NEB Biolabs) to give pJV6. pJV10: this plasmid results from the subcloning of pJV7 (fragment with the spectinomycin resistance cassette flanked by BsaI sites and Ptet-CD2517 toxin) into pJV6 (ErmR) using the restriction enzymes SacII and XhoI. pJV11: the upstream and downstream regions of lcpA were amplified from genomic DNA of 630 strain using respectively JV58/59 and JV62/JV63 primers. ..

    Plasmid Preparation:

    Article Title:
    Article Snippet: .. Both PCR products were assembled using the Gibson assembly protocol (NEB Biolabs) to give pJV6. pJV10: this plasmid results from the subcloning of pJV7 (fragment with the spectinomycin resistance cassette flanked by BsaI sites and Ptet-CD2517 toxin) into pJV6 (ErmR) using the restriction enzymes SacII and XhoI. pJV11: the upstream and downstream regions of lcpA were amplified from genomic DNA of 630 strain using respectively JV58/59 and JV62/JV63 primers. ..

    Article Title: A fluorescent perilipin 2 knock-in mouse model visualizes lipid droplets in the developing and adult brain
    Article Snippet: The sequences covering the sgRNA target site were extracted from the genomic DNA using PCR, run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). tdTomato and neomycin were cloned out from H3.1-miCOUNT (Denoth-Lippuner et al., 2020) using PCR, run on a 1 % agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). .. The plasmid backbone, pFA6 (Janke et al., 2004) was digested using the restriction enzymes SacII (#R0157S, New England Biolabs) and HindIII (#R3104T, New England Biolabs). .. All fragments were cloned together using the Gibson Assembly ® Master Mix (#E2611S, New England Biolabs), therefore all primes used for amplifying the fragments were designed to have an overhang to align to the adjacent fragment in the plasmid.

    Article Title: A fluorescent perilipin 2 knock-in mouse model reveals a high abundance of lipid droplets in the developing and adult brain
    Article Snippet: The sequences covering the sgRNA target site were extracted from the genomic DNA using PCR, run on a 1% agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). tdTomato and neomycin were cloned out from H3.1-miCOUNT (Denoth-Lippuner et al., 2020) using PCR, run on a 1% agarose gel and gel extracted using QIAquick Gel Extraction Kit (#28704, Qiagen). .. The plasmid backbone, pFA6 (Janke et al., 2004) was digested using the restriction enzymes SacII (#R0157S, New England Biolabs) and HindIII (#R3104T, New England Biolabs). .. All fragments were cloned together using the Gibson Assembly® Master Mix (#E2611S, New England Biolabs).

    Subcloning:

    Article Title:
    Article Snippet: .. Both PCR products were assembled using the Gibson assembly protocol (NEB Biolabs) to give pJV6. pJV10: this plasmid results from the subcloning of pJV7 (fragment with the spectinomycin resistance cassette flanked by BsaI sites and Ptet-CD2517 toxin) into pJV6 (ErmR) using the restriction enzymes SacII and XhoI. pJV11: the upstream and downstream regions of lcpA were amplified from genomic DNA of 630 strain using respectively JV58/59 and JV62/JV63 primers. ..

    Amplification:

    Article Title:
    Article Snippet: .. Both PCR products were assembled using the Gibson assembly protocol (NEB Biolabs) to give pJV6. pJV10: this plasmid results from the subcloning of pJV7 (fragment with the spectinomycin resistance cassette flanked by BsaI sites and Ptet-CD2517 toxin) into pJV6 (ErmR) using the restriction enzymes SacII and XhoI. pJV11: the upstream and downstream regions of lcpA were amplified from genomic DNA of 630 strain using respectively JV58/59 and JV62/JV63 primers. ..



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    Reagents and tools table

    Journal: EMBO Reports

    Article Title: High CDC20 levels increase sensitivity of cancer cells to MPS1 inhibitors

    doi: 10.1038/s44319-024-00363-8

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Ndel, SacII, AgeI and EcoRI restriction enzymes , New England Biolabs , R0111S, R0157S, R3552S, R3101S.

    Techniques: Recombinant, Plasmid Preparation, Sequencing, Control, Software, Activity Assay, CRISPR, Knock-Out, Isolation, SYBR Green Assay, Imaging